Deoxyribonucleic Acid Polymerase from Wheat Embryos
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منابع مشابه
Deoxyribonucleic Acid polymerase from wheat embryos.
A soluble DNA-dependent DNA polymerase was extracted from wheat embryos. In vitro, the incorporation of radioactive thymidine triphosphate into acid-insoluble material is dependent upon the presence of the enzyme, all four deoxyribonucleotide triphosphates, Mg(2+), and a DNA template. Incorporation occurs on native, alkali-denatured, and strictly double-stranded DNA. The in vitro synthesized pr...
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Germinating wheat embryos (Triticum vulgare var. Florence) synthesize proteins before the onset of DNA synthesis. The onset of DNA replication occurs at about 15 hours of germination and was shown to depend on proteins synthesized before 9 hours of germination with the use of blasticidin S, a specific inhibitor of protein synthesis. A 10-fold increase in the activity of DNA-dependent DNA polyme...
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Three DNA polymerases (A, B and C) have been purified from the soluble cytoplasm of ungerminated wheat embryos. Mainly on the basis of chromatographic, template-specificity and salt-inhibition evidence, we have characterized the three enzymes. Other physicochemical and enzymic properties are described. From purified mitochondria we have purified a DNA polymerase that behaves like DNA polymerase...
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DNA polymerase III has been purified 4,500-fold from the Escherichia coli mutant, HMS83, which lacks DNA polymerases I and II. When subjected to disc gel electrophoresis, the most purified fraction exhibits a single major protein band from which enzymatic activity may be recovered. Polyacrylamide gel electrophoresis under denaturing conditions produces two protein bands with molecular weights o...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1974
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.53.3.377